Technical Resources empowered at CRBA
* Molecular Biology: PCR amplification Real time PCR Mutation and SNP detection using DHPLC, polarized fluorescence, MALDI-TOF High throughput SNP analysis Automated DNA Sequencing Gel electrophoresis & blotting Gel Imaging Hybridization * Proteomics: Western Blot 2D Gel (shared with CIRB) Gel Cutter & Digest Maldi-TOF ESI-Qtof (shared with CIRB) Protein and metabolites identification by LC/MS/MS * In vitro Biophase: Primary cell cultures Cellular gene transfection Bacterial plasmid cloning * In vivo Biophase: Autoventilated racks for rodent cages Surgery and microsurgey on rodents Isolated Organ perfusion * Fractionation Cell fractionation Sub cellular fractionation Flow citometry – cell sorting * Hystology and cytology: Cyto-Histo preparations Microtomy Cryo-microtomy Enzymatic and molecular cytochemistry Immunocytochemistry In situ hybridization Light microscopy Laser microdissection Image cytometry Microspectrophotometry * Biochemistry: Immunochemistry using multilabeled ELISA Time resolved & fluorescence polarization analyses Spectrometry Gas cromatography - mass spectometry Radiochemistry Gas-cromatography HPLC combined with UV, Fluo & Coulometric detectors * Bio-informatics and Statistics
